Generating and Amplifying Adenoviral Constructs: Difference between revisions
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# Add virus sample (up to 2.5 ml) | # Add virus sample (up to 2.5 ml) | ||
# Elute with 5 ml PBS, discard first 20 drops and collect 10 drop fractions into 10 tubes. | # Elute with 5 ml PBS, discard first 20 drops and collect 10 drop fractions into 10 tubes. | ||
# OD 260 fractions in nanodrop, virus is in middle fractions (5-7 in my extraction). Good virus yield is >3 OD. | # OD 260 fractions in nanodrop, virus is in middle fractions (5-7 in my extraction). Good virus yield is >3 OD. | ||
# dilute to 1 OD/ml | |||
# Add 10% glycerol and store at -80. | # Add 10% glycerol and store at -80. | ||