Glycogen Determination from Tissues: Difference between revisions
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## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. (for microplate add 100ul) | ## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. (for microplate add 100ul) | ||
## Add 1-5 uL glucose standard (500mg/dL) for standard curve (for microplate add 1-5 ul of glucose standard 200mg/dL diluted 1:5) | ## Add 1-5 uL glucose standard (500mg/dL) for standard curve (for microplate add 1-5 ul of glucose standard 200mg/dL diluted 1:5) | ||
## Add 10 uL digested glycogen | ## Add 10 uL digested glycogen (for mice fasted more than 6 hours. for fed/short fast need less) | ||
## Mix and incubate at 37C for 5 min | ## Mix and incubate at 37C for 5 min | ||
## Measure absorbance at 505 nm | ## Measure absorbance at 505 nm | ||