Glycogen Determination from Tissues: Difference between revisions
m changed category |
No edit summary |
||
| Line 25: | Line 25: | ||
# Resuspend pellets in 200uL amyloglucosidase solution and incubate at 37C for 3h-O/N | # Resuspend pellets in 200uL amyloglucosidase solution and incubate at 37C for 3h-O/N | ||
# Quantify glucose using kit: | # Quantify glucose using kit: | ||
## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. | ## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. (for microplate add 100ul) | ||
## Add 1-5 uL glucose standard (500mg/dL) for standard curve | ## Add 1-5 uL glucose standard (500mg/dL) for standard curve (for microplate add 1-5 ul of glucose standard 200mg/dL diluted 1:5) | ||
## Add 10 uL digested glycogen | ## Add 10 uL digested glycogen | ||
## Mix and incubate at 37C for 5 min | ## Mix and incubate at 37C for 5 min | ||