Preparation of RNA Samples from Mouse Tissues: Difference between revisions
m changed category |
changed volume to remove of upper phase (typo from original protocol) |
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#Incubate at room temperature for 2-3 minutes. | #Incubate at room temperature for 2-3 minutes. | ||
#Centrifuge in a cold eppendorf centrifuge on maximum for 15 minutes. Sample will separate into a lower, red chloroform phase, an interphase and a colorless upper phase which contains the RNA. | #Centrifuge in a cold eppendorf centrifuge on maximum for 15 minutes. Sample will separate into a lower, red chloroform phase, an interphase and a colorless upper phase which contains the RNA. | ||
#Transfer | #Transfer 400 uL of the upper phase to a fresh tube. | ||
#Add | #Add 400 uL of 70% ethanol and mix by vortexing. | ||
#Remove 700 uL of mixture (invert briefly to mix before taking sample) and add to a spin column in a collection tube. | #Remove 700 uL of mixture (invert briefly to mix before taking sample) and add to a spin column in a collection tube. | ||
#Spin 15s on max (press button). Discard flow through. Add remaining sample and respin. | #Spin 15s on max (press button). Discard flow through. Add remaining sample and respin. | ||