Immunoprecipitation: Difference between revisions
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* spin once more and aspirate all supernatant. | * spin once more and aspirate all supernatant. | ||
* add 50 ul of sample buffer, boil 5 minutes | * add 50 ul of sample buffer, boil 5 minutes | ||
* to get rid of beads - open cap, prick bottom with 25G needle [[File:Needle_in_eppendorf_2.jpg| | * to get rid of beads - open cap, prick bottom with 25G needle [[File:Needle_in_eppendorf_2.jpg|50px]] (up to halfway of bevel). | ||