Immunofluoresence: Difference between revisions
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#Incubate in 500X secondary solution 45 minutes in 2% BSA PBS | #Incubate in 500X secondary solution 45 minutes in 2% BSA PBS | ||
#Wash coverslips 3 times 10 minutes with PBS with gentle rocking | #Wash coverslips 3 times 10 minutes with PBS with gentle rocking | ||
#Add 10 uL vectashield to glass slide and place cells on slide. Fix with nail polish, dry for at least 1 hour. | #Add 10 uL vectashield to glass slide and place cells on slide (coverslip side up, use sharp tweezers and tip, 2 coverslips on each slide). Label slide with date, cells and treatment + color code for antibodies. Fix with nail polish, dry for at least 1 hour up to ON in dark drawer. Store long term in sealed box at 4C. | ||
*It is possible to use ImageJ to analyse [[Colocalization]] | *It is possible to use ImageJ to analyse [[Colocalization]] | ||
[[Category:Immunofluoresence]] | [[Category:Immunofluoresence]] | ||