Verifying Kan Deletion Mutants from Genomic DNA: Difference between revisions
copied protocol from emily kauffman |
m changed PCR reaction to a list |
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==PCR Reaction== | ==PCR Reaction== | ||
1 uL Genomic DNA | * 1 uL Genomic DNA | ||
2.5 uL Expand Long Template Buffer 1 | * 2.5 uL Expand Long Template Buffer 1 | ||
1.25 uL of a 1:10 dilution of the forward primer | * 1.25 uL of a 1:10 dilution of the forward primer | ||
1.25 uL of a 1:10 dilution of the reverse primer | * 1.25 uL of a 1:10 dilution of the reverse primer | ||
0.5 uL dNTP mix (10mM stock) | * 0.5 uL dNTP mix (10mM stock) | ||
1 uL Expand Long Template Enzyme | * 1 uL Expand Long Template Enzyme | ||
17.5 uL ddH2O | * 17.5 uL ddH2O | ||
25 µl Total Volume | * 25 µl Total Volume | ||
==PCR Program== | ==PCR Program== | ||