Glycogen Determination from Tissues: Difference between revisions
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m described preparation and storage of amyloglucosidase |
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* Ethanol | * Ethanol | ||
* 50 mM Sodium Acetate, pH 4.8 | * 50 mM Sodium Acetate, pH 4.8 | ||
* Amyloglucosidase 0.3 mg/mL | * Amyloglucosidase 0.3 mg/mL in 50 mM Sodium Acetate. Stored in -80 | ||
* Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf) | * Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf) | ||
* Glucose standard solution (200 or 500 mg/dL; Wako) | * Glucose standard solution (200 or 500 mg/dL; Wako) | ||