RNA-seq Pipeline for Known Transcripts: Difference between revisions
added information about using FastQC |
m added link to FastQC report analysis |
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# FASTQC available at http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/ | # FASTQC available at http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/ | ||
## Open run_fastqc on a windows machine. Individually open each sequence file and allow it to analyse. Save this report. | ## Open run_fastqc on a windows machine. Individually open each sequence file and allow it to analyse. Save this report. | ||
## Check this report to decide if sequences need to be trimmed or discarded. | ## Check this report to decide if sequences need to be trimmed or discarded. See http://bioinfo.cipf.es/courses/mda11/lib/exe/fetch.php?media=ngs_qc_tutorial_mda_val_2011.pdf for sample results and an explanation of the quality report | ||
===Filter Sequences Using FastX-Toolkit=== | ===Filter Sequences Using FastX-Toolkit=== | ||