Difference between revisions of "Preparation of Tail Samples (for Genotyping)"
From Bridges Lab Protocols
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Revision as of 14:28, 11 August 2011
PBND Solution: Tail Lysis Buffer -50mM KCl -10mM Tris HCl (pH~8.3) -0.1 mg/mL MgCl2, 6H20 -0.1 mg/mL Gelatin -0.45% (NP-40) Jgepal -0.45% Tween 20
Protocol 1.) Combine 200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube 2.) Incubate at 55 degrees (3hrs-O/N) 3.) Incubate at 85 degrees for 45 min 4.) PCR
