Real Time PCR From Cell Culture: Difference between revisions
altered primer concentration and volumes for 384 well plate |
added reference for calculating amounts from Ct |
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#Using a multichannel pipettor, add 10 uL Master mix to each well. If using a 384 well plate use 5uL | #Using a multichannel pipettor, add 10 uL Master mix to each well. If using a 384 well plate use 5uL | ||
#Start qPCR Machine using the machine in the [[qPCR - Lin Lab|Lin Lab]] or the [[qPRC - Saltiel Lab|Saltiel Lab]] machine | #Start qPCR Machine using the machine in the [[qPCR - Lin Lab|Lin Lab]] or the [[qPRC - Saltiel Lab|Saltiel Lab]] machine | ||
==Calculations== | |||
see http://www.ncbi.nlm.nih.gov/pmc/articles/PMC55695 for considerations on calculations | |||
==References (Saltiel Lab)== | ==References (Saltiel Lab)== | ||