Colocalization: Difference between revisions
Created page with '#Open ImageJ Software #Prior to performing colocalisation analysis save cell images as experiment files #Open an experiment file in ImageJ by File->Open->Select File #Select Fi...' |
updated protocol |
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#Select File and split by Image->color->Split | #Select File and split by Image->color->Split | ||
#Select each image and change to 8-bit greyscale by Image->type->8bit | #Select each image and change to 8-bit greyscale by Image->type->8bit | ||
#Assess colocalisation by Plugins->Colocalisation | #Assess colocalisation by Plugins->Analyze->Colocalisation Finder | ||
For a z-stack | |||
#Open image and change to 8-bit (Image->Type->8 bit) | |||
#Flatten z-stacks by going Image->Z-Project and selecting the range of images (typically the first x images are one color and then the last x images are the next color) | |||
#Select max intensity from the pulldown list | |||
#Repeat for the other images | |||
'''Assessment''' | '''Assessment''' | ||