Protein-Lipid Overlay Assay: Difference between revisions
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# Wash the blots and incubate for 1hr at room temperature with a secondary antibody in 5% non-fat dry milk in TBS with 0.1% Tween20. | # Wash the blots and incubate for 1hr at room temperature with a secondary antibody in 5% non-fat dry milk in TBS with 0.1% Tween20. | ||
# Wash as in (5) & visualize the blot using Super-Signal chemiluminescent kit (Pierce). | # Wash as in (5) & visualize the blot using Super-Signal chemiluminescent kit (Pierce). | ||
[[ Category: Protein-Lipid Interactions ]] | |||
[[ Category: Western Blot ]] | |||