3T3-L1 Plasma Membrane Isolation: Difference between revisions
Created page with '#Wash cells 3x in HES (20 mM Hepes, pH 7.4; 1 mM EDTA, 255 mM Sucrose) #Dry cells well and add 0.6 mL HES (w/Protease Inhibitor) per 150 mm dish #Scrape cells and homogenize with...' |
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