Difference between revisions of "Genotyping Program"

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(Created page with '{| border="1" !| Allele || Program|| Fragment Size(s) || Notes |- |TSC-floxed || Inoki PCR || || Two bands if heterozygote |- |Cre || Inoki PCR || || Only present if Transgene...')
 
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|TC10b || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
 
|TC10b || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
 
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*Bring up to 500 mL with ddH20
 
*Sterile filter buffer and store at 4C
 
 
[[ Category:Genotyping ]]
 
[[ Category:Genotyping ]]
 
[[ Category:Mouse Work]]
 
[[ Category:Mouse Work]]
 
[[ Category:PCR]]
 
[[ Category:PCR]]

Revision as of 15:07, 6 December 2010

Allele Program Fragment Size(s) Notes
TSC-floxed Inoki PCR Two bands if heterozygote
Cre Inoki PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Inoki PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote