CPY Assay: Difference between revisions
Created page with 'Carboxypeptidase Secretion Assay == Protocol == *Revive Yeast Cells (2-3) days *Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast. (Does this have to be sele...' |
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*Primary antibody (polyclonal against cpy location?) how long? | *Primary antibody (polyclonal against cpy location?) how long? | ||
*Secondary antibody (where/what is it and how long?) | *Secondary antibody (where/what is it and how long?) | ||
Wash with pbs in between? | |||
What is ECL? | |||