Dilution Formula: Difference between revisions
Created page with 'C1V1=C2V2 C1=measured absorption V1=unknown volume to be added to final volume V2=desired volume to re-suspend V1 in (usually 4mL) C2=<math>OD/2^x</math> x=amount of times ce...' |
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C1V1=C2V2 | C1V1=C2V2 | ||
C1=measured absorption | *C1=measured absorption | ||
V1=unknown volume to be added to final volume | *V1=unknown volume to be added to final volume | ||
V2=desired volume to re-suspend V1 in (usually 4mL) | *V2=desired volume to re-suspend V1 in (usually 4mL) | ||
C2= | *C2=OD/2^x | ||
x=amount of times cells will double | |||
*x=amount of times cells will double | |||
*find this by dividing the number of hours the cells will be growing by how long one doubling period is | |||