Cesium Chloride Preparation of DNA: Difference between revisions
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*GTE Buffer (50 mM Glucose, 25 mM Tris, 10 mM EDTA) | *GTE Buffer (50 mM Glucose, 25 mM Tris, 10 mM EDTA) | ||
*Lysis Solution (0.2N NaOH, 1% SDS) | *Lysis Solution (0.2N NaOH, 1% SDS) | ||
*Sodium Acetate (pH 4.8 and 3M pH 7) | *Sodium Acetate (3M pH 4.8 and 3M pH 7; pH with Acetic Acid) | ||
*Ultracentrifuge tubes (Beckman 362185) | *Ultracentrifuge tubes (Beckman 362185) | ||
*Water-Saturated N-butanol | *Water-Saturated N-butanol | ||