FM4-64 Labeling of Yeast Cells: Difference between revisions
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==Materials== | ==Materials== | ||
*Instead of FM4-64 we use SynaptoRed (Sigma #S6689). This is resuspended 1mg to 500 uL (2ug/mL) with DMSO | *Instead of FM4-64 we use SynaptoRed (Sigma #S6689). This is resuspended 1mg to 500 uL (2ug/mL) with DMSO and stored in '''Molecular Biology Stuff''' | ||
*Media (see [[YPD Media and Agar]]) | *Media (see [[YPD Media and Agar]]) | ||
*Dye solution. For each cell line need 250 uL media + 3 uL dye. | *Dye solution. For each cell line need 250 uL media + 3 uL dye. | ||