Purification of GST-HA-S6K: Difference between revisions
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#Collect supernatant (save a lysate sample) into a 15 mL Falcon tube | #Collect supernatant (save a lysate sample) into a 15 mL Falcon tube | ||
#Add 0.5 mL Glutatione-sepharose beads and incubate end over end for 1-2h. | #Add 0.5 mL Glutatione-sepharose beads and incubate end over end for 1-2h. | ||
[[Category: Protein Purification]] | |||
[[Category: mTOR]] | |||
[[Category: Kinase Assay]] | |||