PCR Amplification of DNA (KOD Polymerase): Difference between revisions
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*dNTPs – dilute to 2 mM each in water, make single use aliquots (50 uL aliquots... 10uL of each dNTP, 460uL water) | *dNTPs – dilute to 2 mM each in water, make single use aliquots (50 uL aliquots... 10uL of each dNTP, 460uL water) | ||
*Template – generally 1uL or less of a plasmid miniprep | *Template – generally 1uL or less of a plasmid miniprep | ||
*Polymerase | *KOD Polymerase | ||
==Protocol== | ==Protocol== | ||