Preparing Cell Lysates: Difference between revisions
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! || Final Concentration || per 10 mL || Stock | ! || Final Concentration || per 10 mL || Stock | ||
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|Tris pH7.4 || 50mM || 500uL || 1M | |Tris pH7.4 || 50mM || 500uL || 1M (pH 8.0@25oC) | ||
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|Na Deoxycholate || 0.25% || 250uL || 10% | |Na Deoxycholate || 0.25% || 250uL || 10% | ||
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|EDTA || 1mM || 20uL || 0.5M | |EDTA || 1mM || 20uL || 0.5M | ||
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|NaVO3 || | |NaVO3 || 100uM || 10uL || 100mM | ||
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|NaF || 5mM || 100uL || | |NaF || 5mM || 100uL || 0.5M | ||
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|NaPPi || 25 mM || 1 mL || | |NaPPi || 25 mM || 1 mL || 250mM | ||
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|- Protease Inhibitors !! 1 tab | |- Protease Inhibitors !! 1 mini tab | ||
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