Preparation of DIG Labelled Probes: Difference between revisions
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*Probe blot for >4h with DIG-probe, wash and use secondary at 10 000 X dilute in 5% SMP | *Probe blot for >4h with DIG-probe, wash and use secondary at 10 000 X dilute in 5% SMP | ||
*Visualize with ECL | *Visualize with ECL | ||
[[Category: Blotting]] | |||
[[Category: Protein Purification]] | |||
[[Category: Protein-Protein Interactions]] | |||