Restriction Enzyme Based Cloning - Ordering Primers: Difference between revisions
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#Check restriction sites either with NEBCutter (http://tools.neb.com/NEBcutter2/index.php) | #Check restriction sites either with NEBCutter (http://tools.neb.com/NEBcutter2/index.php) | ||
#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp). For example: | #If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp). For example: | ||
::BamHI = BclI = BglII | ::BamHI = BclI = BglII | ||
::EcoRI = MfeI = ApoI | ::EcoRI = MfeI = ApoI | ||
::XbaI = AvrII = SpeI = NheI | ::XbaI = AvrII = SpeI = NheI | ||