Colony PCR: Difference between revisions
Created page with '==Reagents== *Primers *dNTPs (2 mM of each dNTP) *Expand polymerase (Roche) *Primers *Yeast Colonies ==Protocol== #Pick about 20% of a colony and mix in 20 uL of sterile water. ...' |
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#4C hold forever | #4C hold forever | ||
*Check PCR by Agarose Gel Electrophoresis | *Check PCR by Agarose Gel Electrophoresis | ||
[[Category:Cloning]] | |||
[[Category:PCR]] | |||
[[Category:Genotyping]] | |||
[[Category:Yeast]] | |||