Restriction Enzyme Based Cloning - Ordering Primers: Difference between revisions
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#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp). | #If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp). | ||
#For example: | #For example: | ||
::BamHI = BclI = BglII; | |||
::EcoRI = MfeI = ApoI | |||
::XbaI = AvrII = SpeI = NheI | |||
::XhoI = SalI = PspXI | |||
==Design primers== | ==Design primers== | ||
#Design 3' end of primers with NCBI Primer Blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast) or Primer3Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi). | #Design 3' end of primers with NCBI Primer Blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast) or Primer3Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi). | ||