Restriction Enzyme Based Cloning - Ordering Primers: Difference between revisions

added ncbi primer blast
mNo edit summary
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#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp).   
#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp).   
#For example:
#For example:
**BamHI = BclI = BglII;  
::BamHI = BclI = BglII;  
**EcoRI = MfeI = ApoI
::EcoRI = MfeI = ApoI
**XbaI = AvrII = SpeI = NheI
::XbaI = AvrII = SpeI = NheI
**XhoI = SalI = PspXI
::XhoI = SalI = PspXI
==Design primers==
==Design primers==
#Design 3' end of primers with NCBI Primer Blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast) or Primer3Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi).
#Design 3' end of primers with NCBI Primer Blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast) or Primer3Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi).