Designing and Preparing dsRNA: Difference between revisions
mNo edit summary |
mNo edit summary |
||
| Line 13: | Line 13: | ||
===Template Preparation=== | ===Template Preparation=== | ||
*set up a 250 uL PCR reaction | *set up a 250 uL PCR reaction | ||
** | **25 uL 10X KOD buffer | ||
**50 uL | **25 uL dNTPs | ||
** | **50 uL Primers (sense and antisense combined and diluted to 1 uM. 2 uL of each primer plus 196 uL of water) | ||
**15 uL MgCl (this can be adjusted to optimize PCR conditions) | |||
**Template (previously prepared PCR product or 1 uL of cDNA) | **Template (previously prepared PCR product or 1 uL of cDNA) | ||
**2 uL KOD polymerase | **2 uL KOD polymerase | ||