Differentiation of 3T3-L1 Cells: Difference between revisions
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*Good cells should be homogeneous and have >10 fold insulin stimulated glucose uptake. | *Good cells should be homogeneous and have >10 fold insulin stimulated glucose uptake. | ||
*If adipocytes need to be trypsinized for replating or electroporation this is best done before the fourth day after changing to Adipocyte media. Use 0.25% trypsin. After that, cells do not trypsinize well. | *If adipocytes need to be trypsinized for replating or electroporation this is best done before the fourth day after changing to Adipocyte media. Use 0.25% trypsin. After that, cells do not trypsinize well. | ||
==Reference== | |||
<pubmed>16472699</pubmed> | |||