Culturing S2 Cells: Difference between revisions
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==Materials== | ==Materials== | ||
*'''S2 cells''' | *'''S2 cells''' see [[Freezing and Thawing S2 Cells]] | ||
*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs | *'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs | ||
*'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs | *'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs | ||
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#Add 10 mL to fresh 10 cm dishes | #Add 10 mL to fresh 10 cm dishes | ||
#Add 1 mL of detached cells to each new plate | #Add 1 mL of detached cells to each new plate | ||
==Knockdown== | |||
*[[Designing and Preparing dsRNA]] | |||
*[[dsRNA Mediated Knockdown in S2 Cells]] | |||
==References== | ==References== | ||