Culturing S2 Cells: Difference between revisions

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+==Materials==
+*'''S2 cells''' see [[Freezing and Thawing S2 Cells]]
+*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs
+*'''Penicillin/streptomycin''' 50X  mix (Invitrogen, cat. no. 15240062) - stocked downstairs
+*'''Insect Cell Media'''.  Filter together Media (500 mL) and Pen/Strep (10 mL) into a tissue culture bottle
+==Protocol==
+*Typically cells are split every 3-4 days at a ratio of 1:5 and grown at 25C
+*After 2-3 days the cells become slightly less adherent.
+#To split, gently aspirate the media (some cells will have detached from the plate surface)
+#Pipet a gentle stream of media over the surface of the plate (5 mL for normal passage)
+#Add 10 mL to fresh 10 cm dishes
+#Add 1 mL of detached cells to each new plate
+==Knockdown==
+*[[Designing and Preparing dsRNA]]
+*[[dsRNA Mediated Knockdown in S2 Cells]]
 ==References==
 see PMID 11752672 and PMID 18388942
 [[Category: Cell Culture]]
 [[Category: Drosophila]]
-[[Category: Knockdown]]