Culturing S2 Cells: Difference between revisions
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==Materials== | |||
*'''S2 cells''' see [[Freezing and Thawing S2 Cells]] | |||
*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs | |||
*'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs | |||
*'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep (10 mL) into a tissue culture bottle | |||
==Protocol== | |||
*Typically cells are split every 3-4 days at a ratio of 1:5 and grown at 25C | |||
*After 2-3 days the cells become slightly less adherent. | |||
#To split, gently aspirate the media (some cells will have detached from the plate surface) | |||
#Pipet a gentle stream of media over the surface of the plate (5 mL for normal passage) | |||
#Add 10 mL to fresh 10 cm dishes | |||
#Add 1 mL of detached cells to each new plate | |||
==Knockdown== | |||
*[[Designing and Preparing dsRNA]] | |||
*[[dsRNA Mediated Knockdown in S2 Cells]] | |||
==References== | ==References== | ||
see | see PMID 11752672 and PMID 18388942 | ||
[[Category: Cell Culture]] | |||
[[Category: Drosophila]] | |||