Western Blotting: Difference between revisions
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==Materials== | ==Materials== | ||
*Protein Markers, we use SeaBlue Plus2 from Invitrogen (cat #LC5925) | |||
*Nitrocellulose, we use BioRad 0.45 um membrane from a roll (cat #1620115) and cut to the size of a gel | |||
*Filter Paper, we use BioRad extra thick paper (cat 1703966) | |||
*Transfer Buffer (200 mL Methanol, 100 mL 10X [[ Transfer Buffer ]] to final 1L volume) | *Transfer Buffer (200 mL Methanol, 100 mL 10X [[ Transfer Buffer ]] to final 1L volume) | ||
*Transfer Apparatus, either Bio-Rad or Invitrogen | *Transfer Apparatus, either Bio-Rad or Invitrogen | ||