Determining Cortisol Levels in Human Hair: Difference between revisions
→Procedure: copied procedure in from Jiayin, from the manual |
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* Draw out a 96 well grid (8 x 12) labelling where you intend to put the blanks, standards, | * Draw out a 96 well grid (8 x 12) labelling where you intend to put the blanks, standards, | ||
==== Addition of the reagents | ==== Addition of the reagents ==== | ||
* Add 100 µl ELISA Buffer (1X) to NSB wells. | * Add 100 µl ELISA Buffer (1X) to NSB wells. | ||
* Add 50 µl ELISA Buffer (1X) to blank wells. | * Add 50 µl ELISA Buffer (1X) to blank wells. | ||