Real Time PCR From Cell Culture: Difference between revisions
→RT-PCR Reaction: added details about RT Reaction |
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===RT-PCR Reaction=== | ===RT-PCR Reaction=== | ||
#Book PCR Machine for 2h | #Book PCR Machine for 2h | ||
#Thaw, mix and quickly spin RNA, dNTP mix, random hexamers, 10X RT buffer, 25 mM MgCl2 water | #Thaw, mix and quickly spin RNA, dNTP mix, random hexamers, 10X RT buffer, 25 mM MgCl2 water. All reagents are in the small red invitrogen box | ||
#Combine the following: | #Combine the following in a PCR tube: | ||
##8 uL RNA | ##8 uL RNA | ||
##1 uL dNTP mix | ##1 uL dNTP mix | ||