Buffer/RIPA: Difference between revisions
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mistake with NaF and NaPPi concentrations. Added note about NaVO3 location |
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==RIPA Buffer (for 10mL lysis buffer)== | ==RIPA Buffer (for 10mL lysis buffer)== | ||
{| border="1" | {| border="1" | ||
! || Final Concentration || per 10 mL || Stock | ! || Final Concentration || per 10 mL || Stock || Location | ||
|- | |- | ||
|Tris pH7.4 || 50mM || 500uL || 1M | |Tris pH7.4 || 50mM || 500uL || 1M | ||
| Line 13: | Line 13: | ||
|EDTA || 1mM || 20uL || 0.5M | |EDTA || 1mM || 20uL || 0.5M | ||
|- | |- | ||
|NaVO3 || 1mM || 100uL || 1M | |NaVO3 || 1mM || 100uL || 1M || Biochemical Stocks (-20) | ||
|- | |- | ||
|NaF || 5mM || 100uL || | |NaF || 5mM || 100uL || 500mM | ||
|- | |- | ||
|NaPPi || 25 mM || 1 mL || | |NaPPi || 25 mM || 1 mL || 250mM | ||
|- | |- | ||
| | | Protease Inhibitors || || 1 mini tablet || || 4C | ||
|} | |} | ||