Fugene Transfection of 293T/COS Cells: Difference between revisions
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#Add Fugene to OptiMEM, incubate ~5 min. | #Add Fugene to OptiMEM, incubate ~5 min. | ||
#Add required amount of DNA to eppendorf tubes. | #Add required amount of DNA to eppendorf tubes. | ||
#Add 51 uL | #Add 51 uL OptiMEM/Fugene per ug DNA to each DNA tube. | ||
#Incubat 5-20 min. | #Incubat 5-20 min. | ||
#Split confluent cells 2-3X into fresh dishes as follows: | #Split confluent cells 2-3X into fresh dishes as follows: | ||