Surface Plasmon Resonance - Protein Lipid Interactions: Difference between revisions
m Additions from protocol handwritten by Dave. |
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==Preparation of Liposomes== | ==Preparation of Liposomes== | ||
#Combine DOPC + C16 lipid (or DOPC alone) at a ratio of 97:3 and dry under nitrogen ( | #Combine DOPC + C16 lipid (or DOPC alone) at a ratio of 97:3 and dry under nitrogen (15uL PC or 14.5uL PC + 0.5 uL PI) | ||
#Resuspend to 10 mM total lipid with HBS-N (100 uL). Vortex thoroughly and sonicate in water bath | #Resuspend to 10 mM total lipid with HBS-N (100 uL). Vortex thoroughly and sonicate in water bath | ||
#Correct pH to 7.4 using pH paper and 0.6uL aliquots of 1M NaOH | #Correct pH to 7.4 using pH paper and 0.6uL aliquots of 1M NaOH | ||
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*Tightly attach syringe and place on blcok. | *Tightly attach syringe and place on blcok. | ||
*Pass 250uL of milliQ water through the filger ~5 times. | *Pass 250uL of milliQ water through the filger ~5 times. | ||
*Discard the water and inject the sample through the filter 10 times. | *Discard the water and inject the sample through the filter 10 times. | ||
==Preparation of Surface== | ==Preparation of Surface== | ||