Immunofluoresence: Difference between revisions
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#Treat cells as required | #Treat cells as required | ||
#Wash cells twice with 2 mL cold PBS then fix for 10 min at RT | #Wash cells twice with 2 mL cold PBS then fix for 10 min at RT | ||
Use neutral buffered formalin, 4% paraformaldehyde in PHEM or ice cold 10% methanol | #Use neutral buffered formalin, 4% paraformaldehyde in PHEM or ice cold 10% methanol | ||
#Wash twice with PBS | #Wash twice with PBS | ||
#Add 200 uL of 100mM Glycine in PBSfor 5 min to quench | #Add 200 uL of 100mM Glycine in PBSfor 5 min to quench | ||