Milk Creamatocrit: Difference between revisions
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==Mouse Milk Creamatocrit (milk fat %)== | ==Mouse Milk Creamatocrit (milk fat %)== | ||
This method is done to assess the total fat content of mouse milk. It does not differentiate between type of fat present. Additional information on mouse milk composition can be ascertained through triglyceride or cholesterol assay, or through lipidomics. | |||
===Materials=== | ===Materials=== | ||
*Milk samples (from -80) | *Milk samples (from -80) | ||
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* After the 8th spin, immediately read the creamatocrit using the calipers | * After the 8th spin, immediately read the creamatocrit using the calipers | ||
===Reading the samples=== | ===Reading the samples=== | ||
* Measure to the nearest 0.01 inch the aqueous layer, the fat layer, and the small aqueous layer on top of the fat layer. | * Measure each of the 3 layers to the nearest 0.01 inch with the calipers. Record the number in the corresponding columns of [https://docs.google.com/spreadsheets/d/e/2PACX-1vTm1Zo5Cf9RTuw_uRwum8h_dOBb0mjc5HYcOHR1nBPHKbSzxlFqwYfJs0NMdChFHrczE_8sKCU3K9lp/pub?output=csv the spreadsheet](the aqueous layer(Aqueous.inches.1), the fat layer(Fat.inches), and the small aqueous layer on top of the fat layer(Aqueous.inches.2)). | ||
* Calculate the milk fat percentage using the following R code: | * Calculate the milk fat percentage using the following R code: | ||