Changes

* Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen.

** Tamoxifen takes a long time to go into solution so plan to prepare ahead of time.

* Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together.

* To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL.

* Tamoxifen can be stored at room temperature for up to a week.

* The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically.