PCR Amplification of DNA: Difference between revisions
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==Protocol== | ==Protocol== | ||
*Use the following volumes per reaction | *Use the following volumes per reaction | ||
::* | ::*12.5 uL DreamTaq | ||
::* | ::*7.5 uL RNAse-Free water | ||
::*5.0 uL Working stock Primer (of 0.4-1uM stock solution in water (both primers combined)) | |||
==PCR Program== | |||
: | *For animals: use program denoted in [[Genotyping Program]] | ||
*Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''DAVETD''') as follows: | *Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''DAVETD''') as follows: | ||