4EBP1/2 Genotyping: Difference between revisions
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*4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X) | *4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X) | ||
*4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X) | *4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X) | ||
WT= 175bp KO= 550bp | |||
'''4EBP2''' | '''4EBP2''' | ||
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*4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X) | *4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X) | ||
*4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X) | *4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X) | ||
WT= 450bp KO= 750bp | |||
Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately. | Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately. | ||