Mc4r-Cone Genotyping: Difference between revisions
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!| PCR Step || Temperature || Time || Cycles || Notes | !| PCR Step || Temperature || Time || Cycles || Notes | ||
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1. Initiation/Melting || 94 || 5:00 || 1 || | |1. Initiation/Melting || 94 || 5:00 || 1 || | ||
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2. Denaturation || 94 || 0:45 || 40 || | |2. Denaturation || 94 || 0:45 || 40 || | ||
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3. Annealing || 66 || 0:45 || 40 || | |3. Annealing || 66 || 0:45 || 40 || | ||
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4. Elongation || 72 || 0:45 || 40 || steps 2-4 to be run in sequence | |4. Elongation || 72 || 0:45 || 40 || steps 2-4 to be run in sequence | ||
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5. Amplification || 72 || 7:00 || 1 || | |5. Amplification || 72 || 7:00 || 1 || | ||
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6. Finish || 4 || infinite || n/a || | |6. Finish || 4 || infinite || n/a || | ||
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