Freezing and Thawing S2 Cells: Difference between revisions

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added SOP requirements-JR
 
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==SOP==
*[[SOP- Cryogenic Materials]]
*[[SOP- Biosafety Cabinets]]
==METHOD==
#Use and early passage (1-10) of exponentially growing (about ~10^7 cells).
#Use and early passage (1-10) of exponentially growing (about ~10^7 cells).
#Detach by pipeting a stream of media over the cells
#Detach by pipeting a stream of media over the cells