Preparing a SDS-PAGE Gel: Difference between revisions
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==SOP== | |||
*[[SOP- Electrophoresis]] | |||
==Separating Gel== | ==Separating Gel== | ||
*For 12 mL final volume (2 gels) | *For 12 mL final volume (2 gels) | ||
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| TEMED || 30 uL || 30 uL || 30 uL || 30uL | | TEMED || 30 uL || 30 uL || 30 uL || 30uL | ||
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*For a Phos-Tag Gel, add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl in the separating gel only | *For a [[Phos-Tag Electrophoresis and Western Blotting|Phos-Tag Gel]], add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl in the separating gel only | ||
==Stacking Gel== | ==Stacking Gel== | ||
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[[Category: SDS-PAGE]] | [[Category: SDS-PAGE]] | ||
[[Category: Electrophoresis]] | [[Category: Electrophoresis]] | ||
[[Category: PhosTag]] | |||
[[Category: Phosphorylation]] | |||