Difference between revisions of "Dexamethasone Treatment of Mice"
From Bridges Lab Protocols
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* average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L | * average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L | ||
<pre> | <pre> | ||
| − | 65mg dex/ = 6mg dex/ => X= 0.092g | + | 65mg dex/ = 6mg dex/ => X= 0.092g solid powder |
| − | 1000mg solid powder ? (X) mg powder | + | 1000mg solid powder ? (X) mg solid powder |
</pre> | </pre> | ||
*This assumes that the mice are about 30g and they drink 5mL/day. | *This assumes that the mice are about 30g and they drink 5mL/day. | ||
Latest revision as of 20:26, 30 December 2019
We use water-soluble Dex from Sigma (D2915). It says on the bottle how many mg/g is actually Dex. The calculations below are for a bottle where the powder was 65mg/g. Dex
Contents
For i.p. injection 5 mg/kg
- Makes 15mL stock solution of 1mg/mL
- dissolve 0.231g Dex powder in 15mL PBS
BW (g) X 0.005 = injection volume (mL)
Calculation
65mg dex/ = 15mg dex/ = 0.231g 1000mg powder ? mg powder
For drinking water administration ~1mg/kg/day
- Makes 1L of drinking water
- dissolve 0.092g in 1L water
Calculation
- 1mg Dex/kg/day because mice drink ~5mL /day so want 0.2 mg Dex/kg/mL
- average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
65mg dex/ = 6mg dex/ => X= 0.092g solid powder 1000mg solid powder ? (X) mg solid powder
- This assumes that the mice are about 30g and they drink 5mL/day.
