Triglyceride Assay from Cells and Tissues: Difference between revisions
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#Vortex vigorously then sit at room temperature for 5 minutes | #Vortex vigorously then sit at room temperature for 5 minutes | ||
#Centrifuge for 10 minutes @ 13000G | #Centrifuge for 10 minutes @ 13000G | ||
#Transfer | #Transfer 200 ul of the bottom layer into a new tube | ||
#Centrifuge again for 7-10 minutes @ 13000G | |||
#Transfer 150 ul of the bottom layer into the new tube (if there is enough of the bottom layer, transfer a total of 200 ul) | |||
#Let evaporate overnight at room temperature | #Let evaporate overnight at room temperature | ||
#Add'''(50ul)''' of '''Butanol Mixture''' and vortex. See Suggested Volumes for your specific tissue. | #Add'''(50ul)''' of '''Butanol Mixture''' and vortex. See Suggested Volumes for your specific tissue. | ||