Ketone Body Tolerance Test: Difference between revisions
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*Prepare a 1 g/10mL solution of glucose in the unlikely case that some animals become hyperketonemic. | *Prepare a 1 g/10mL solution of glucose in the unlikely case that some animals become hyperketonemic. | ||
*Weigh/MRI mice ahead of time, mark tails if necessary with different colors for rapid identification and take fasting ketone measurement via a tail clip. | *Weigh/MRI mice ahead of time, mark tails if necessary with different colors for rapid identification and take fasting ketone measurement via a tail clip. | ||
*Prepare bOHB syringes with 1.5 g/KG mouse weight (ie for a | *Prepare bOHB syringes with 1.5 g/KG mouse weight (ie for a mouse with 15g lean body mass, use 15uL). | ||
*At approximately 1 min intervals, inject appropriate amount of bOHB into intraperitoneal cavity of the mouse. | *At approximately 1 min intervals, inject appropriate amount of bOHB into intraperitoneal cavity of the mouse. | ||
**Immobilize mouse and restrain tail with one hand. | **Immobilize mouse and restrain tail with one hand. | ||
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*Analyze data by both % change from fasting ketones and absolute values. Our preferred outcome is to report fasting ketone levels and report percent change as a figure. | *Analyze data by both % change from fasting ketones and absolute values. Our preferred outcome is to report fasting ketone levels and report percent change as a figure. | ||
*The preferred statistical model is a mixed linear model using the time points as ordinate values and testing for a main effect or an interaction of the treatment/genotype. Use the R lme4 package for this. | *The preferred statistical model is a mixed linear model using the time points as ordinate values and testing for a main effect or an interaction of the treatment/genotype. Use the R lme4 package for this. | ||
[[Category:Ketogenesis]] | [[Category:Ketogenesis]] | ||