QPCR: Difference between revisions
added link to google calendar |
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===Plate Preparation=== | ===Plate Preparation=== | ||
#Book 2h on qPCR machine by signing up on the sheet in room 7013 | #Book 2h on qPCR machine by signing up on the sheet in room 7013 and on the [https://calendar.google.com/calendar/embed?src=umich.edu_jkmcg58uidmngdh0sutt1d36og%40group.calendar.google.com&ctz=America%2FDetroit google calendar] | ||
#Prepare cDNA and dilute in water in a 96 well plate. Typically a 20x dilution of cDNA leaves enough to be detected. | #Prepare cDNA and dilute in water in a 96 well plate. Typically a 20x dilution of cDNA leaves enough to be detected. | ||
#Get optically clear 384 well plate and keep on paper towel. Do not touch bottom of plate. | #Get optically clear 384 well plate and keep on paper towel. Do not touch bottom of plate. | ||