QPCR Analysis on ThermoCloud: Difference between revisions

Line 7:

**Verify that each of your replicates amplified for each target.

**If there is a target that yields 2+ peaks over all samples, mark that target as unusable in the '''Primer Database'''.

**Exclude samples that did not the above criteria by clicking the well not the plate schematic and '''Omit''' under the '''Action''' dropdown box above the plate schematic.

**Exclude samples that did not pass the above criteria by clicking the well (not the plate schematic) and select '''Omit''' under the '''Action''' dropdown box above the plate schematic.

*Check the technical replicates by choosing the '''Table View''' and arranging them according to target. Verify that the '''CT''' values have <1.5 difference. Omit outliers.

*Click '''Next''' and '''Export''' your results to the appropriate project folder.

@@ Line 7: / Line 7: @@
 **Verify that each of your replicates amplified for each target.
 **If there is a target that yields 2+ peaks over all samples, mark that target as unusable in the '''Primer Database'''.
-**Exclude samples that did not the above criteria by clicking the well not the plate schematic and '''Omit''' under the '''Action''' dropdown box above the plate schematic.
+**Exclude samples that did not pass the above criteria by clicking the well (not the plate schematic) and select '''Omit''' under the '''Action''' dropdown box above the plate schematic.
 *Check the technical replicates by choosing the '''Table View''' and arranging them according to target.  Verify that the '''CT''' values have <1.5 difference.  Omit outliers.
 *Click '''Next''' and '''Export''' your results to the appropriate project folder.